Journal: bioRxiv
Article Title: Inexpensive High-Throughput Multiplexed Cytokine Detection for Tuberculosis Diagnostics Using Amplified Enzymatic Metallization
doi: 10.64898/2026.01.27.700981
Figure Lengend Snippet: (a) Schematic of laser-cut PDMS microwell array reversibly assembled on a polystyrene petri dish and workflow of enzymatically amplified silver metallization using Biotin-BSA and poly-HRP-SA. (b) Cell-phone image of silver metallized wells after assay. (c) Biotin-BSA dilution curve obtained from cell-phone image silver darkness quantification in ImageJ. (d) Assay stack for initial biotinyl tyramide signal amplification testing using biotinylated anti-IFN-γ as capture. (e) Dilution curve of silver darkness of increasing biotinylated anti-IFN-γ as target and 10 µg/mL, 1 µg/mL, and 0 µg/mL biotinyl tyramide. All assays were repeated three (n=3) times and fit lines are four-parameter logistic sigmoidal curves.
Article Snippet: 1-Step Ultra TMB-ELISA Substrate Solution (34029), Poly-HRP Streptavidin (N200), HRP-Conjugated Streptavidin (N100), RPMI-1640 Medium (A1049101), Fetal Bovine Serum (A5670401), Penicillin-Streptomycin (15140122), and Phytohemagglutinin-L (PHA-L) Solution (00-4977-93) were obtained from Thermo Fisher Scientific.
Techniques: Amplification